Hitme // World · Living Planetary Instrument
WARP 7.0 0.0° N, 0.0° E
OUTERMOST LIVING INSTRUMENT
Continents: Thin Glass · Stars: 16-Byte Atoms
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Sickbay // Cathedral Window & Plain PADD
=== THE SICKBAY CATHEDRAL PADD === "Sickbay is not a hospital in this picture. It is a window that prints a padd when someone is brave enough to ask a bench for help. The padd is plain. The window is cathedral. Wonder lives in the contrast." --- 1-PAGE WET-LAB ASSAY WORK ORDER (BENCH-READY) --- Study: Raft Disruption vs Direct P-gp Block in Paclitaxel-Resistant TNBC Hypothesis: C_raft ↓ ==> J_P-gp ↓ ==> F(t=1h) ↑ ==> IC50(231-PacR) ↓ Host Gate : MCF-10A viability >= 80% at tumor IC50 exposure 1. CELL LINES: • MDA-MB-231-PacR (Acquired Paclitaxel-Resistant TNBC) • MDA-MB-231-P (Parental Taxane-Sensitive Control) • MCF-10A (Non-Malignant Human Mammary Host Gate) 2. 3 REQUIRED BENCH PLOTS: [Plot 1] J_P-gp Efflux Surrogate : Rhodamine-123 / Calcein-AM Flow Cytometry (Fold vs vehicle) [Plot 2] F(t*) Free Drug Accum : 100 nM Paclitaxel, 1.0 h, LC-MS/MS Intracellular Peak Area [Plot 3] 72-hr IC50 Dose Curve : 8-Point Serial Dilution (0.1–1000 nM) with MCF-10A Gate Full Protocol: https://gotokell.hitme.dev/docs/cro_methods_sheet.md TSV Plate Map: https://gotokell.hitme.dev/docs/pilot_plate_map.tsv
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